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Trichoderma reesei Rut C-30 produced high levels of β-glucosidase, endo-β-1,4-glucanase, and exo-β-1,4-glucanase. In pilot-scale production (50-1 fermentor), productivity and yield of CMCase (carborymethyl cellulose) and FPase (filter paper activity) were 273U/ml and 35U/ml, and 162FPU/l · h and 437FPU/g, respectively. The fed-batch techniques were used to improve enzyme activities with constant cell concentration. The acidity was an important parameter and controlled at pH 3.9 and 5.0 by automatic addition of ammonium hydroxide. Cellulase powder was prepared by ammonium sulfate precipitation and its CMCase and FPase activities were 3,631U/g and 407U/g, respectively.
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